On completion of the course, students should:1. Demonstrate knowledge and understanding of the principles of Flow Cytometry (FC).2. Be able to obtain and critically assess FC data using specific analysis software applications and pre-acquired samples.3. Become familiar with instruments and their components, demonstrate understanding and critical awareness of the process of analysis protocol creation.4. Integrate knowledge of good laboratory practice in instrument usage, sample preparation, quality control, troubleshooting.5. Describe the biology of apoptosis, the cell cycle & general physiology.6. Be able to integrate knowledge of apoptosis, cell cycle and physiology into protocol design for FC analysis and to carry out sample analysis using standard (and/or own) samples and protocols.7. Understand the concepts of immunophenotyping & phagocytosis.8. Apply knowledge and understanding of FC to the analysis of immunological & phagocytic cells, carry out sample characterization using standard (and/or own) samples and protocols.9. Become critically aware of uses of FC outside the academic research setting.10. Achieve understanding of allied technologies such as high content analysis, live cell imaging, confocal and fluorescent microscopy and be able to critically assess the synergistic benefits of flow cytometric analysis in combination with allied technologies.

Not applicable to this module.