CHEN30250 Biochemical Engineering Lab 2

Academic Year 2020/2021

This is a core module for the BE in Chemical Engineering with Biochemical Engineering Minor. The purpose of the module is to provide students with hands-on experience of the equipment and utilities associated with a typical biopharmaceutical production facility. Practical sessions will take place over 3 days, during Spring, in the National Institute for Bioprocessing Research & Training (NIBRT). Students, working in pairs/groups will scheduled for a series of practical sessions, on which they will report, via technical reports and associated assignments. Activities will be selected from the following:

- introduction to the NIBRT facilities, including gowning protocols
- contamination control
- basic cell culture techniques, including use of laminar air flow cabinets
- upstream processing: bioreactor instrumentation & steam-in-place (SIP), disposable systems
- downstream processing: centrifugation, tangential-flow filtration (TFF), process-scale ion-exchange chromatography (equipment, column packing, asymmetry testing)

A laboratory manual will be provided.
In preparing for laboratory sessions and in preparing reports and assignments, students are expected to consult the relevant literature, including industry standards and regulations and relevant CHEN module materials.

This module will also include lectures (and associated exercises) on experimental design principles and on the principles of operations/techniques of relevance to process/analytical applications.

An alternative linear mark-to-grade conversion system (40% pass) is used in this module for grades of C- and above (i.e. C-: 55.00-59.99; C: 60.00-64.99; C+65:00-69.99; B-:70.00-74.99; B: 75.00-79.99; B+: 80.00-84.99; A-: 85.00-89.99; A: 90.00-94.99; A+:95.00-100.00).


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Curricular information is subject to change

Learning Outcomes:

On completion of this module the student should:

1. Have an applied knowledge of the equipment and utilities associated with a typicial biopharmaceutical production facility.
2. Appreciate the need for asepetic technique and sterility in a typicial biopharmaceutical facility.
3. Be able to identify and explain the role of the main components of a fully-instrumented bioreactor.
4. Be able to explain the role of disposables in biopharmaceutical manufacture and perform standard connection operations.
5. Have undertaken a complete in-place sterilization of a bioreactor and auxiliary equipment.
6. Be familiar with the basic techniques for working with CHO cells, for the preparation of bioreactor inocula.
7. Be familiar with large-scale chromatography equipment, including column packing and column testing for symmetry.
8. Be familiar with selected centrifugation and filtration equipment, for biopharmacuetical downstream processing operations.
9. Be able to apply the principles of experimental design.

Student Effort Hours: 
Student Effort Type Hours
Lectures

8
Laboratories

24
Specified Learning Activities

10
Autonomous Student Learning

80
Total

122
Approaches to Teaching and Learning:
Laboratory work; peer and group work; lectures; critical writing; student presentations, etc. 
Requirements, Exclusions and Recommendations

Not applicable to this module.


Module Requisites and Incompatibles
Not applicable to this module.
 
Assessment Strategy  
Description Timing Open Book Exam Component Scale Must Pass Component % of Final Grade
Continuous Assessment: Reports & Assignments Varies over the Trimester n/a Alternative linear conversion grade scale 40% No

100

Carry forward of passed components
Yes
 
Resit In Terminal Exam
Summer Yes - 1 Hour
Please see Student Jargon Buster for more information about remediation types and timing. 
Feedback Strategy/Strategies

• Feedback individually to students, post-assessment
• Online automated feedback
• Peer review activities
• Self-assessment activities

How will my Feedback be Delivered?

Not yet recorded.

Name Role
Dr Roderick Jones Lecturer / Co-Lecturer